Validated using a knockout cell line
Recombinant
RabMAb

Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

Overview

  • Product name
    Anti-Rab4 antibody [EPR3043] - Early Endosome Marker
    See all Rab4 primary antibodies
  • Description
    Rabbit monoclonal [EPR3043] to Rab4 - Early Endosome Marker
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Rab4 aa 150-250 (C terminal).

  • Positive control
    • MCF7, PC12, Neuro 2a, 293T, SH SY5Y and Human fetal brain lysates; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab109009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
IP 1/10 - 1/100.
ICC/IF 1/250 - 1/1000.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    Images



    • Predicted band size : 24 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Rab4 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Human fetal brain lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109009 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab109009 was shown to specifically react with Rab4 when Rab4 knockout samples were used. Wild-type and Rab4 knockout samples were subjected to SDS-PAGE. ab109009 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW)  preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)  preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labelling Rab4 with purified ab109009 at 1/250. Cells were fixed with 100% methanol and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • All lanes : Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : PC12 cell lysate
      Lane 3 : Neuro 2a cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : SH SY5Y cell lysate
      Lane 6 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 24 kDa
    • ab109009 at 1/500 dilution staining Rab4 in HeLa by Immunofluorescence.

    References

    This product has been referenced in:
    • Niu Y  et al. Ablation of SNX6 leads to defects in synaptic function of CA1 pyramidal neurons and spatial memory. Elife 6:N/A (2017). Read more (PubMed: 28134614) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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