The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/12500.
RelevanceRAB40B is similar to a yeast protein which suggests a role of the gene product in regulating secretory vesicles. It may be a substrate recognition component of a SCF like ECS (Elongin Cullin SOCS box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Cellular localizationCell membrane; Lipid anchor; Cytoplasmic side