The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: 1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
Detection Antibody Pair
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionProbable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family. Contains 1 SOCS box domain.
DomainThe SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.