Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746)
Key features and details
- Mouse monoclonal [3A4] to Rab5A - Early Endosome Marker
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Rab5A antibody [3A4] - Early Endosome Marker
See all Rab5A primary antibodies -
Description
Mouse monoclonal [3A4] to Rab5A - Early Endosome Marker -
Host species
Mouse -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Rab5a aa 1-250.
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Positive control
- WB: Extracts of mouse and human brain. ICC/IF: U87MG cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3A4 -
Myeloma
Sp2/0 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). |
Target
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Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cell membrane. Early endosome membrane. Melanosome. Cytoplasmic vesicle. Cell projection, ruffle. Membrane. Cytoplasm, cytosol. Cytoplasmic vesicle, phagosome membrane. Enriched in stage I melanosomes (PubMed:17081065). Alternates between membrane-bound and cytosolic forms (Probable). - Information by UniProt
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Database links
- Entrez Gene: 5868 Human
- SwissProt: P20339 Human
- Unigene: 475663 Human
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Alternative names
- RAB5A antibody
- RAB5A_HUMAN antibody
- Ras-related protein Rab-5A antibody
Images
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Rab5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Hu brain whole cell lysate (20 µg)
Lane 4: Ms brain whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab66746 observed at 25 kDa. Red - loading control, ab181602, observed at 37 kDa.This image was produced using the ascites version of this antibody.
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Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/500 dilution + mouse brain at 45 µg
Secondary
goat anti-mouse secondary antibody conjugated to HRP
Predicted band size: 24 kDa -
Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/500 dilution + extracts of mouse brain at 45 µg
Secondary
HRP-conjugated goat anti-mouse
Developed using the ECL technique.
Predicted band size: 24 kDa
Observed band size: 24 kDaThis image was produced using the ascites version of this antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab66746 has been referenced in 9 publications.
- Patel M et al. Spatial expression of IKK-alpha is associated with a differential mutational landscape and survival in primary colorectal cancer. Br J Cancer 126:1704-1714 (2022). PubMed: 35173303
- Tang Y et al. The effect of drug loading and multiple administration on the protein corona formation and brain delivery property of PEG-PLA nanoparticles. Acta Pharm Sin B 12:2043-2056 (2022). PubMed: 35847504
- Gorji-Bahri G et al. RAB5A effect on metastasis of hepatocellular carcinoma cell line via altering the pro-invasive content of exosomes. Exp Mol Pathol 120:104632 (2021). PubMed: 33831402
- Gorji-Bahri G et al. RAB5A is associated with genes involved in exosome secretion: Integration of bioinformatics analysis and experimental validation. J Cell Biochem 122:425-441 (2021). PubMed: 33225526
- Polla DL et al. Biallelic variants in TMEM222 cause a new autosomal recessive neurodevelopmental disorder. Genet Med 23:1246-1254 (2021). PubMed: 33824500
- Nanou A et al. Endothelial Tpl2 regulates vascular barrier function via JNK-mediated degradation of claudin-5 promoting neuroinflammation or tumor metastasis. Cell Rep 35:109168 (2021). PubMed: 34038728
- Huang HW & Ryoo HD Drosophila fabp is required for light-dependent Rhodopsin-1 clearance and photoreceptor survival. PLoS Genet 17:e1009551 (2021). PubMed: 34714826
- Winer H et al. Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins. Nat Commun 9:3744 (2018). PubMed: 30218067
- Del Cid-Pellitero E et al. Homeostatic Changes in GABA and Glutamate Receptors on Excitatory Cortical Neurons during Sleep Deprivation and Recovery. Front Syst Neurosci 11:17 (2017). PubMed: 28408870