Anti-Rab5 antibody [3A4] (ab66746)

Overview

  • Product nameAnti-Rab5 antibody [3A4]
    See all Rab5 primary antibodies
  • Description
    Mouse monoclonal [3A4] to Rab5
  • Tested applicationsSuitable for: WB, ELISA, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to amino acids 1-215 of Human Rab5 purified from E. coli.

  • Positive control
    • Extracts of mouse brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab66746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 2-5µg for 106 cells.

Target

Anti-Rab5 antibody [3A4] images

  • ab66746 staining Rab5 in the U87MG human cell line at a dilution of 1/100. 

    DAPI was used for nuclear staining.

  • Anti-Rab5 antibody [3A4] (ab66746) at 1/500 dilution + extracts of mouse brain at 45 µg

    Secondary
    HRP-conjugated goat anti-mouse
    Developed using the ECL technique

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
  • Flow cytometry analysis of ab66746 staining Rab5 in HeLa cells. Primary antibody was used at a concentration of 2-5ug per 1 X 106 cells. The secondary antibody used was an Alexa fluor 488® conjugated goat anti-mouse IgG.

References for Anti-Rab5 antibody [3A4] (ab66746)

ab66746 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step None
Permeabilization No
Specification Brain
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 6% · Temperature: 20°C
Fixative Formaldehyde
Username

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Verified customer

Submitted Aug 09 2016

Thank you for your email.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab66746.
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Thank you for contacting us.

Yes, I'd be happy to send you one of the mouse antibodies, but am afraid I cannot make the final decision for you. I'd be able to make a recommendation, though.
Both antibodies (ab66746,ab55657) have not bee...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"