Overview

  • Product name
    Anti-Rab5 antibody - C-terminal
    See all Rab5 primary antibodies
  • Description
    Rabbit polyclonal to Rab5 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Rab5 aa 182-211 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. NP_004153.2
    Sequence:

    PKNEPQNPGANSARGRGVDLTEPTQPTRNQ


    Database link: P20339

  • Positive control
    • Human prostate and brain tissues; HL60 cells; HL60 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab190215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 24 kDa.
IHC-P 1/50 - 1/100.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/10 - 1/50.

Target

  • Function
    Required for the fusion of plasma membranes and early endosomes.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization
    Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • RAB 5 antibody
    • RAB 5A antibody
    • RAB5A antibody
    • RAB5A member RAS oncogene family antibody
    • RAB5A_HUMAN antibody
    • RAS associated protein RAB5A antibody
    • Ras related protein Rab 5A antibody
    • Ras-related protein Rab-5A antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human prostate tissue labeling Rab5 with ab190215 at 1/50 dilution.

  • Immunofluorescence on paraffin-embedded Human brain tissue labeling Rab5 with ab190215 at 25 μg/ml, followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. Red counterstaining is PI.

  • Flow cytometry of HL60 cells (right histogram) compared to a negative control cell (left histogram), labeling Rab5 with ab190215 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Anti-Rab5 antibody - C-terminal (ab190215) at 1/1000 dilution + HL60 cell line lysate at 35 µg

    Predicted band size: 24 kDa

References

ab190215 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab190215.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up