Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Fruit fly (Drosophila melanogaster) Rab5.
(Peptide available as ab32489.)
Our Abpromise guarantee covers the use of ab31261 in the following tested applications.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20404143|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).|
ab31261 staining 3rd instar wing imaginal disc cells of fruit fly (Drosophila melanogaster). Cells were formaldehyde fixed and blocked with 5 % serum for 2 hours at 22°C prior to incubating with the primary antibody, diluted 1/500, for 16 hours at 4°C. A Cy3® conjugated donkey anti-rabbit IgG (H+L) antibody, diluted 1/500, was used as the secondary.
In the image, the nuclei of some cells are marked by GFP (green). The punctate, non-nuclear staining pattern is indicative of endosomes (similar to other endosomal markers such as Hrs).