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Product Name
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Rab5 antibody - Drosophila Early Endosome Marker
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See all Rab5 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Rab5 - Drosophila Early Endosome Marker
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Note on antibody grade |
Fast-Track antibodies
- Immunogen affinity purified
- high ELISA titre values
- released early (to accelerate your research)
- 950 Abpoints to the 1st researcher
who submits conclusive data (positive or negative):
Read the Fast-Track antibody Terms of Use
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Fruit fly (Drosophila melanogaster) Rab5. (Peptide available as ab32489.)
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Reacts with
(species key)
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Dm
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Abreviews
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Application notes
(see key)
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Recommended dilutions
This antibody gave a positive result in ELISA against the immunizing peptide (ab32489).
A customer has used this successfully in ICC/IF at 1:500 dilution. Please see abreview.
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Cellular localization
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Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome.
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Research areas
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Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Rabs
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Relevance
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Rab5 is a 24 kDa GTP-binding protein that regulates the fusion of plasma membrane-derived clathrin-coated vesicles with early endosomes and homotypic fusion among early endosomes. It is localized to the cytoplasmic side of the plasma membrane, clathrin coated vesicles and early endosomes. Rab5 is believed to regulate vesicle fusion through a cycle of GDP/GTP exchange and GTP hydrolysis. The different guanine nucleotide binding states of Rab5 is postulated to affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. Its GTP-bound form, which represents the active form of Rab5, associates with membrane and regulates vesicle docking and fusion. Studies using a Rab5 mutant that hydrolysed xanthosine 5'-triphosphate (XTP) indicated that nucleotide hydrolysis occurs even in the absence of membrane fusion. GTP hydrolysis by Rab5 is postulated to determine the frequency of membrane docking and fusion events.
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.10 - 1.00 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rab5 antibody - Drosophila Early Endosome Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rab5 antibody - Drosophila Early Endosome Marker images, references, products related to ab31261 and other tools.
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Rab5 antibody - Drosophila Early Endosome Marker images:
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This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Please read the definition of 'Fast-Track antibody'
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 Immunocytochemistry/ Immunofluorescence - Rab5 antibody - Drosophila Early Endosome Marker (ab31261)
ab31261 staining 3rd instar wing imaginal disc cells of fruit fly (Drosophila melanogaster). Cells were formaldehyde fixed and blocked with 5 % serum for 2 hours at 22°C prior to incubating with the primary antibody, diluted 1/500, for 16 hours at 4°C. A Cy3® conjugated donkey anti-rabbit IgG (H+L) antibody, diluted 1/500, was used as the secondary. In the image, the nuclei of some cells are marked by GFP (green). The punctate, non-nuclear staining pattern is indicative of endosomes (similar to other endosomal markers such as Hrs). This image is courtesy of an anonymous Abreview
See Abreview
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Search PubMed (MEDLINE) for references to Rab5
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Rab5 antibody - Drosophila Early Endosome Marker - more information
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Customer reviews (feedback) regarding Rab5 antibody - Drosophila Early Endosome Marker |
Customer FAQs regarding Rab5 antibody - Drosophila Early Endosome Marker |
Protocols for Rab5 antibody - Drosophila Early Endosome Marker |
Price and availability of products related to Rab5 antibody - Drosophila Early Endosome Marker |
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Rab5 antibody - Drosophila Early Endosome Marker - related products:
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Rab5 antibody - Drosophila Early Endosome Marker - other tools:
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Contact Abcam with a Technical Enquiry about Rab5 antibody - Drosophila Early Endosome Marker |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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