Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261)

Overview

  • Product name
    Anti-Rab5 antibody - Drosophila Early Endosome Marker
    See all Rab5 primary antibodies
  • Description
    Rabbit polyclonal to Rab5 - Drosophila Early Endosome Marker
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Fruit fly (Drosophila melanogaster) Rab5.

    (Peptide available as ab32489.)

  • Positive control
    • This antibody gave a positive signal in Schneider L2 (Fruit fly (Drosophila melanogaster)) Whole Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab31261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20404143
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

Target

  • Function
    Required for the fusion of plasma membranes and early endosomes.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization
    Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes.
  • Information by UniProt
  • Alternative names
    • RAB 5 antibody
    • RAB 5A antibody
    • RAB5A antibody
    • RAB5A member RAS oncogene family antibody
    • RAB5A_HUMAN antibody
    • RAS associated protein RAB5A antibody
    • Ras related protein Rab 5A antibody
    • Ras-related protein Rab-5A antibody
    see all

Images

  • ab31261 staining 3rd instar wing imaginal disc cells of fruit fly (Drosophila melanogaster).  Cells were formaldehyde fixed and blocked with 5 % serum for 2 hours at 22°C prior to incubating with the primary antibody, diluted 1/500, for 16 hours at 4°C.  A Cy3® conjugated donkey anti-rabbit IgG (H+L) antibody, diluted 1/500, was used as the secondary.

    In the image, the nuclei of some cells are marked by GFP (green).  The punctate, non-nuclear staining pattern is indicative of endosomes (similar to other endosomal markers such as Hrs).

    See Abreview

  • Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261) at 1 µg/ml + Schneider L2 whole cell lysate (ab14893) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
    Additional bands at : 135 kDa,145 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ab31261 staining Rab5 in Drosophila S2 cells by Immunocytochemistry/ Immunofluorescence.
    S2 cells were fixed in 4% paraformaldehyde for 10 minutes and then permeablized by incubating with 0.2% Triton X-100 in PBS for 5 minutes at room temperature. After blocking, cells were incubated overnight with ab31261 diluted in PBS containing 10% horse serum and 0.2% Triton X-100. A Fluorescein-conjugated anti-rabbit IgG was used as the secondary.

References

This product has been referenced in:
  • Shimizu H  et al. The Drosophila ZO-1 protein Polychaetoid suppresses Deltex-regulated Notch activity to modulate germline stem cell niche formation. Open Biol 7:N/A (2017). IHC (PFA fixed) ; Drosophila melanogaster . Read more (PubMed: 28424321) »
  • Vonk JJ  et al. Drosophila Vps13 Is Required for Protein Homeostasis in the Brain. PLoS One 12:e0170106 (2017). WB . Read more (PubMed: 28107480) »

See all 27 Publications for this product

Customer reviews and Q&As

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Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 25% · Temperature: 23°C
Sample
Fruit fly (Drosophila melanogaster) Tissue (Pupae, notum, epithelial)
Specification
Pupae, notum, epithelial
Permeabilization
Yes - PBST
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Fruit fly (Drosophila melanogaster) Cell (larval fat body)
Specification
larval fat body
Permeabilization
Yes - PBS + 0.1% Triton X-100
Fixative
Paraformaldehyde
Username

Dr. Anne-Claire Jacomin

Verified customer

Submitted Aug 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (whole third instar larvae)
Specification
whole third instar larvae
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Fruit fly (Drosophila melanogaster) Cell (wing)
Specification
wing
Fixative
Paraformaldehyde
Permeabilization
Yes - NP40
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 14 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Fruit fly (Drosophila melanogaster) Cell (Eye disc)
Specification
Eye disc
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Apr 29 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Fruit fly (Drosophila melanogaster) Cell (3rd instar wing imaginal disc)
Specification
3rd instar wing imaginal disc
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 23 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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