Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

Overview

  • Product nameAnti-Rab5 antibody [EPR5438] - Early Endosome Marker
    See all Rab5 primary antibodies
  • Description
    Rabbit monoclonal [EPR5438] to Rab5 - Early Endosome Marker
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Rab5 aa 150-250.

  • Positive control
    • 293T, HeLa and Jurkat cell lysates; Human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab109534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    Anti-Rab5 antibody [EPR5438] - Early Endosome Marker images

    • All lanes : purified at 1/5000 dilution

      Lane 1 : Mouse brain
      Lane 2 : Rat brain

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 24 kDa
      Observed band size : 24 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/5000 dilution (purified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : HEK293 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 24 kDa
      Observed band size : 24 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemical staining of paraffin embedded human pancreas with purified ab109534 at a working dilution of 1 in 1000. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/1000 dilution (unpurified)

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 24 kDa
    • Immunohistochemical staining of Rab5 in paraffin embedded human brain tissue with unpurified ab109534, at 1/100 dilution.

    References for Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

    This product has been referenced in:
    • Chen S  et al. Identification of an Essential Region for Translocation of Clostridium difficile Toxin B. Toxins (Basel) 8:N/A (2016). Read more (PubMed: 27537911) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (Pancreatic Cancer)
    Gel Running Conditions Reduced Denaturing (12.5%)
    Loading amount 30 µg
    Specification Pancreatic Cancer
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 01 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HepaRG)
    Specification HepaRG
    Fixative Formaldehyde
    Permeabilization Yes - 0.1% TritonX-100
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Username

    Mrs. Alina Macovei

    Verified customer

    Submitted May 16 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HepaRG)
    Loading amount 20 µg
    Specification HepaRG
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Username

    Mrs. Alina Macovei

    Verified customer

    Submitted May 15 2013

    The IgG concentration of Abcam ab109534 lot GR68319 (which is the same as Rab5a 3768-1 Epitomics lot YH112425) is 0.032 mg/ml. This is lower than the IgG concentration of most mouse monoclonal and rabbit polyclonal antibodies but is not unusual for rab...

    Read More

    The IgG concentrations of the current lots are:

    3768-1 (ab109534, lot GR68319) - 0.032 mg/ml

    3660-1 (ab125068, lot GR75050) - 1.554 mg/ml

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"