Rab5 antibody [Rab5-65] (ab50523)

Product Name 

Rab5 antibody [Rab5-65]

Product type 

Primary antibodies

Description 

Mouse monoclonal [Rab5-65] to Rab5

Immunogen 

Synthetic peptide: ASRGATRPNGPNT conjugated to KLH, corresponding to amino acids 2-14 of Human Rab5
BLAST 'ASRGATRPNGPNT' with or

Reacts with 

Hu, Ms, Rat, Cat, Dog

Predicted to react 
(species key)

Chk, Cow, CynMk, Pig
(Due to sequence homology)

Tested applications 
(see key)

I-ELISA, ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
I-ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Array: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Total cell extract of HeLa cells

Cellular localization 

Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome.

Research areas 

Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Rabs

Relevance 

Rab5 is a 24 kDa GTP-binding protein that regulates the fusion of plasma membrane-derived clathrin-coated vesicles with early endosomes and homotypic fusion among early endosomes. It is localized to the cytoplasmic side of the plasma membrane, clathrin coated vesicles and early endosomes. Rab5 is believed to regulate vesicle fusion through a cycle of GDP/GTP exchange and GTP hydrolysis. The different guanine nucleotide binding states of Rab5 is postulated to affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. Its GTP-bound form, which represents the active form of Rab5, associates with membrane and regulates vesicle docking and fusion. Studies using a Rab5 mutant that hydrolysed xanthosine 5'-triphosphate (XTP) indicated that nucleotide hydrolysis occurs even in the absence of membrane fusion. GTP hydrolysis by Rab5 is postulated to determine the frequency of membrane docking and fusion events.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

Rab5-65

Myeloma 

NS1

Isotype 

IgG3

Purity 

IgG fraction

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 2.40 - 2.40 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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