Overview

  • Product nameAnti-RAB6A antibody
    See all RAB6A primary antibodies
  • Description
    Mouse monoclonal to RAB6A
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: DDVRTERGSD VIIMLVGNKT DLADKRQVSI EEGERKAKEL NVMFIETSAK AGYNVKQLFR RVAAALPGME STQDRSREDM IDIKLEKPQE QPVSEGGCSC , corresponding to amino acids 109-208 of Human RAB6A

Properties

Applications

Our Abpromise guarantee covers the use of ab55660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 3 µg/ml.

Target

  • FunctionProtein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
  • Cellular localizationGolgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GTP binding protein RAB6A' antibody
    • GTP binding protein RAB6B antibody
    • Oncogene RAB6 antibody
    • Rab 6 antibody
    • Rab GTPase antibody
    • Rab-6 antibody
    • RAB6 antibody
    • RAB6 member RAS oncogene family antibody
    • RAB6A antibody
    • RAB6A member RAS oncogene family antibody
    • Rab6A variant 3 antibody
    • RAB6A' antibody
    • RAB6A_HUMAN antibody
    • RAB6B antibody
    • Ras related protein Rab 6A antibody
    • Ras-related protein Rab-6A antibody
    • RCO4 3 antibody
    see all

Anti-RAB6A antibody images

  • RAB6A antibody (ab55660) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.


  • Predicted band size : 24 kDa
    RAB6A antibody (ab55660) at 1ug/lane + HL-60 cell lysate at 25ug/lane.
  • ICC/IF image of ab55660 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55660, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-RAB6A antibody (ab55660)

This product has been referenced in:
  • Petrosyan A  et al. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res 12:1704-16 (2014). Read more (PubMed: 25086069) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"