Overview

  • Product nameAnti-RAB6A antibody
    See all RAB6A primary antibodies
  • Description
    Rabbit polyclonal to RAB6A
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Zebrafish
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 178 of RAB6A

  • Positive control
    • H1299 whole cell lysate HeLa cells MDA-MB-468 xenograft tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab95954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

Anti-RAB6A antibody images

  • Anti-RAB6A antibody (ab95954) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 24 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab95954 at 1:200 dilution (1) and merged with DNA probe (2).
  • ab95954, at a 1:500 dilution, staining human RAB6A in MDA-MB 468 xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

References for Anti-RAB6A antibody (ab95954)

This product has been referenced in:
  • Petrosyan A  et al. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res 12:1704-16 (2014). Read more (PubMed: 25086069) »
  • Petrosyan A & Cheng PW A non-enzymatic function of Golgi glycosyltransferases: mediation of Golgi fragmentation by interaction with non-muscle myosin IIA. Glycobiology 23:690-708 (2013). Read more (PubMed: 23396488) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 35 µg
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Sample Rat Cell lysate - other (Rat hepatocytes plasma membrane fraction)
Specification Rat hepatocytes plasma membrane fraction
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Oct 29 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample Rat Cell (Rat hepatocytes)
Specification Rat hepatocytes
Permeabilization No
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Oct 28 2014

Application Western blot
Loading amount 35 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (LNCaP prostate cancer cells)
Specification LNCaP prostate cancer cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Nov 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Panc-1)
Specification Panc-1
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Dec 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"