Overview

  • Product name
    Anti-RAB6A antibody
  • Description
    Rabbit polyclonal to RAB6A
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Zebrafish
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 178 of RAB6A

  • Positive control
    • H1299 whole cell lysate HeLa cells MDA-MB-468 xenograft tissue
  • General notes

    We have not tested the cross reactivity to RAB6B. ab95954 is raised against a full length RAB6A recombinant protein and may cross-react with RAB6B.

Properties

Applications

Our Abpromise guarantee covers the use of ab95954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

Images

  • Anti-RAB6A antibody (ab95954) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 24 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab95954 at 1:200 dilution (1) and merged with DNA probe (2).
  • ab95954, at a 1:500 dilution, staining human RAB6A in MDA-MB 468 xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

References

This product has been referenced in:
  • Hou X  et al. Rab6a is a novel regulator of meiotic apparatus and maturational progression in mouse oocytes. Sci Rep 6:22209 (2016). Read more (PubMed: 26915694) »
  • Petrosyan A  et al. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res 12:1704-16 (2014). Read more (PubMed: 25086069) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
35 µg
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Sample
Rat Cell lysate - other (Rat hepatocytes plasma membrane fraction)
Specification
Rat hepatocytes plasma membrane fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Oct 29 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Rat Cell (Rat hepatocytes)
Specification
Rat hepatocytes
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Oct 28 2014

Application
Western blot
Loading amount
35 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (LNCaP prostate cancer cells)
Specification
LNCaP prostate cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Nov 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Panc-1)
Specification
Panc-1
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Dec 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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