Overview

  • Product nameAnti-RAB7 antibody
    See all RAB7 primary antibodies
  • Description
    Rabbit polyclonal to RAB7
  • Tested applicationsSuitable for: IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Chicken, Cow, Dog, Human, Xenopus laevis, Chimpanzee, Monkey, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a portion of the amino acids 90-140 of Human RAB7A

  • Positive control
    • Skeletal muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab77993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 22781172
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P Use a concentration of 10 µg/ml.

Target

  • FunctionKey regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation.
  • Tissue specificityWidely expressed; high expression found in skeletal muscle.
  • Involvement in diseaseDefects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B) [MIM:600882]; also known as hereditary motor and sensory neuropathy II (HMSN2). CMT2B is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2B is clinically characterized by marked distal muscle weakness and a high frequency of foot ulcers, infections and amputations of the toes. CMT2B inheritance is autosomal dominant.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
  • Cellular localizationLate endosome. Lysosome. Cytoplasmic vesicle > phagosome. Melanosome. Cytoplasmic vesicle > phagosome membrane. Co-localizes with OSBPL1A at the late endosome. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium.
  • Information by UniProt
  • Database links
  • Alternative names
    • CMT2B antibody
    • PRO2706 antibody
    • PSN antibody
    • RAB7, member RAS oncogene family antibody
    • RAB7A antibody
    • RAB7A, member RAS oncogene family antibody
    • RAB7A_HUMAN antibody
    • Ras associated protein RAB7 antibody
    • Ras related protein Rab7 antibody
    • Ras related protein Rab7a antibody
    • Ras-related protein Rab-7a antibody
    see all

Anti-RAB7 antibody images

  • ab77993 at 10µg/ml staining RAB7 in skeletal muscle tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
  • All lanes : Anti-RAB7 antibody (ab77993) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
    Additional bands at : 34 kDa,49 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

References for Anti-RAB7 antibody (ab77993)

This product has been referenced in:
  • Sathyanesan M  et al. A molecular characterization of the choroid plexus and stress-induced gene regulation. Transl Psychiatry 2:e139 (2012). IHC-Fr ; Rat . Read more (PubMed: 22781172) »
  • Baird NL  et al. Arenavirus infection induces discrete cytosolic structures for RNA replication. J Virol 86:11301-10 (2012). Read more (PubMed: 22875974) »

See all 3 Publications for this product

Product Wall

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Application Immunocytochemistry/ Immunofluorescence
Sample Chicken Cell (chicken embryonic epithelial cell)
Specification chicken embryonic epithelial cell
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Tween
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
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Ms. Chyong-Yi Wu

Verified customer

Submitted Feb 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"