Overview

  • Product name
    Anti-RAB7 antibody [Rab7-117]
    See all RAB7 primary antibodies
  • Description
    Mouse monoclonal [Rab7-117] to RAB7
  • Tested applications
    Suitable for: ICC/IF, IP, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Dog, Human, Pig, Monkey, Zebrafish
    Predicted to work with: Rabbit, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    EQAFQTIARNALKQE

    , corresponding to amino acids 163-177 of Human RAB7

  • Positive control
    • HeLa total cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab50533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IP Use at an assay dependent dilution.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 23 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation.
  • Tissue specificity
    Widely expressed; high expression found in skeletal muscle.
  • Involvement in disease
    Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B) [MIM:600882]; also known as hereditary motor and sensory neuropathy II (HMSN2). CMT2B is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2B is clinically characterized by marked distal muscle weakness and a high frequency of foot ulcers, infections and amputations of the toes. CMT2B inheritance is autosomal dominant.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization
    Late endosome. Lysosome. Cytoplasmic vesicle > phagosome. Melanosome. Cytoplasmic vesicle > phagosome membrane. Co-localizes with OSBPL1A at the late endosome. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium.
  • Information by UniProt
  • Database links
  • Alternative names
    • CMT2B antibody
    • PRO2706 antibody
    • PSN antibody
    • RAB7, member RAS oncogene family antibody
    • RAB7A antibody
    • RAB7A, member RAS oncogene family antibody
    • RAB7A_HUMAN antibody
    • Ras associated protein RAB7 antibody
    • Ras related protein Rab7 antibody
    • Ras related protein Rab7a antibody
    • Ras-related protein Rab-7a antibody
    see all

Anti-RAB7 antibody [Rab7-117] images

  • ICC/IF image of ab50533 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50533, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-RAB7 antibody [Rab7-117] (ab50533) at 1/2000 dilution + HepG2 whole cell lysate at 1/2000 dilution

    Secondary
    HRP-conjugated donkey anti-mouse IgG polyclonal at 1/1 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa


    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by Armen Petrosyan

    Blocked with 5% milk for 1 hour at 22°C.

    Incubated with the primary antibody for 12 hour at 4°C in PBS + 1% BSA.

    See Abreview

  • ab50533 staining RAB7 in HepG2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in PBST + 1% donkey serum) for 3 hours at 22°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Anti-RAB7 antibody [Rab7-117] (ab50533) at 1/2000 dilution + Rat hepatocytes whole cell lysate at 20 µg

    Secondary
    HRP-conjugated donkey anti-mouse IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa


    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by Armen Petrosyan

    Blocked with 5% milk for 1 hour at 22°C.

    Incubated with the primary antibody for 12 hour at 4°C in PBS + 1% BSA.

    See Abreview

  • Anti-RAB7 antibody [Rab7-117] (ab50533) at 1/1000 dilution + Lysate prepared from mouse neuroblastoma cells at 10 µg

    Secondary
    HRP-conjugated goat monoclonal to mouse IgG at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 23 kDa


    Exposure time : 20 seconds

    This image is a courtesy of Anonymous Abreview

    See Abreview

  • Anti-RAB7 antibody [Rab7-117] (ab50533) at 1/500 dilution (for 3 hours at 20°C) + Human brain whole tissue lysate at 15 µg

    Secondary
    An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at 20°C

    See Abreview

References for Anti-RAB7 antibody [Rab7-117] (ab50533)

This product has been referenced in:
  • Spencer B  et al. Reducing Endogenous a-Synuclein Mitigates the Degeneration of Selective Neuronal Populations in an Alzheimer's Disease Transgenic Mouse Model. J Neurosci 36:7971-84 (2016). IHC-FrFl ; Mouse . Read more (PubMed: 27466341) »
  • Dolat L & Spiliotis ET Septins promote macropinosome maturation and traffic to the lysosome by facilitating membrane fusion. J Cell Biol 214:517-27 (2016). Read more (PubMed: 27551056) »

See all 26 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (Xenopus laevis - tailbud stage)
Gel Running Conditions
Reduced Denaturing (gel 15%)
Loading amount
20 µg
Specification
Xenopus laevis - tailbud stage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Philipp Vick

Verified customer

Submitted Dec 22 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse macrophage cells)
Permeabilization
No
Specification
mouse macrophage cells
Blocking step
BSA as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 30 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (neuronal cell line)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
30 µg
Treatment
alpha synuclein
Specification
neuronal cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Oct 12 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2 cells)
Permeabilization
Yes - 0.2% Triton X-100
Specification
HepG2 cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Jul 27 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Jul 27 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Loading amount
25 µg
Specification
HepG2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Jul 27 2015

Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Loading amount
20 µg
Specification
Rat hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Jul 27 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample
Human Cell (Ovarian carcinoma (A2780))
Specification
Ovarian carcinoma (A2780)
Permeabilization
Yes - 0.1% Triton-x100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 18 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Cell (Primary mouse hippocampal neurons)
Specification
Primary mouse hippocampal neurons
Permeabilization
Yes - 0.25 % TX 100
Fixative
Paraformaldehyde
Username

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Dec 09 2013

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Pig Cell lysate - whole cell (Pig retinal pigment epithelium (RPE) cells)
Specification
Pig retinal pigment epithelium (RPE) cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Dec 09 2013

1-10 of 29 Abreviews or Q&A

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