Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533)
Key features and details
- Mouse monoclonal [Rab7-117] to RAB7 - Late Endosome Marker
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat, Dog, Human
- Isotype: IgG2b
Overview
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Product name
Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker
See all RAB7 primary antibodies -
Description
Mouse monoclonal [Rab7-117] to RAB7 - Late Endosome Marker -
Host species
Mouse -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Dog, Human
Predicted to work with: Rabbit, Chicken, Cow, Xenopus laevis, Monkey -
Immunogen
Synthetic peptide:
EQAFQTIARNALKQE
, corresponding to amino acids 163-177 of Human RAB7 -
Positive control
- WB: HeLA, HepG2, NRK , NIH3/T3 and MDCK cell lysates. ICC: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
Rab7-117 -
Myeloma
NS1 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC | (1) |
Use at an assay dependent concentration.
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WB | (18) |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 23 kDa.
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Notes |
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ICC
Use at an assay dependent concentration. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 23 kDa. |
Target
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Function
Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. -
Tissue specificity
Widely expressed; high expression found in skeletal muscle. -
Involvement in disease
Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B) [MIM:600882]; also known as hereditary motor and sensory neuropathy II (HMSN2). CMT2B is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2B is clinically characterized by marked distal muscle weakness and a high frequency of foot ulcers, infections and amputations of the toes. CMT2B inheritance is autosomal dominant. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Late endosome. Lysosome. Cytoplasmic vesicle > phagosome. Melanosome. Cytoplasmic vesicle > phagosome membrane. Co-localizes with OSBPL1A at the late endosome. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium. - Information by UniProt
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Database links
- Entrez Gene: 416016 Chicken
- Entrez Gene: 509970 Cow
- Entrez Gene: 404007 Dog
- Entrez Gene: 7879 Human
- Entrez Gene: 19349 Mouse
- Entrez Gene: 29448 Rat
- Entrez Gene: 446841 Xenopus laevis
- Omim: 602298 Human
see all -
Alternative names
- CMT2B antibody
- PRO2706 antibody
- PSN antibody
see all
Images
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All lanes : Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : NRK (rat cell kidney line) cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 5 : MDCK (dog kidney cell line) cell lysate
Secondary
All lanes : Goat Anti-Mouse IgG-peroxidase
Predicted band size: 23 kDa -
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling RAB7 with ab50533 at 10 μg/mL. Cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% saponin. Goat Anti-Mouse IgG, Atto-488 conjugate was used as the secondary antibody.
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Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) at 1/2000 dilution + HepG2 whole cell lysate at 1/2000 dilution
Secondary
HRP-conjugated donkey anti-mouse IgG polyclonal at 1/1 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Exposure time: 30 secondsBlocked with 5% milk for 1 hour at 22°C.
Incubated with the primary antibody for 12 hour at 4°C in PBS + 1% BSA.
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ab50533 staining RAB7 in HepG2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in PBST + 1% donkey serum) for 3 hours at 22°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
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Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) at 1/2000 dilution + Rat hepatocytes whole cell lysate at 20 µg
Secondary
HRP-conjugated donkey anti-mouse IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Exposure time: 30 secondsBlocked with 5% milk for 1 hour at 22°C.
Incubated with the primary antibody for 12 hour at 4°C in PBS + 1% BSA.
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Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) at 1/1000 dilution + Lysate prepared from mouse neuroblastoma cells at 10 µg
Secondary
HRP-conjugated goat monoclonal to mouse IgG at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 20 seconds
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Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) at 1/500 dilution (for 3 hours at 20°C) + Human brain whole tissue lysate at 15 µg
Secondary
An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Blocking Step: 5% Milk for 1 hour at 20°C
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (107)
ab50533 has been referenced in 107 publications.
- Deluco B & Wilson HL Assessment of intestinal macromolecular absorption in young piglets to pave the way to oral vaccination: preliminary results. Vet Res Commun 46:79-91 (2022). PubMed: 34559380
- Patel M et al. Spatial expression of IKK-alpha is associated with a differential mutational landscape and survival in primary colorectal cancer. Br J Cancer 126:1704-1714 (2022). PubMed: 35173303
- Mishra S et al. The Alzheimer's gene SORL1 is a regulator of endosomal traffic and recycling in human neurons. Cell Mol Life Sci 79:162 (2022). PubMed: 35226190
- Park SS et al. Temsirolimus Enhances Anti-Cancer Immunity by Inducing Autophagy-Mediated Degradation of the Secretion of Small Extracellular Vesicle PD-L1. Cancers (Basel) 14:N/A (2022). PubMed: 36077620
- Yang C et al. SMAD3 promotes autophagy dysregulation by triggering lysosome depletion in tubular epithelial cells in diabetic nephropathy. Autophagy 17:2325-2344 (2021). PubMed: 33043774