Overview

  • Product name
    Anti-RAB8A antibody [EPR14873]
    See all RAB8A primary antibodies
  • Description
    Rabbit monoclonal [EPR14873] to RAB8A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RAB8A aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P61006

  • Positive control
    • HepG2, HeLa, 293T, HCT-116, C6, Raw264.7, PC-12 and NIH3T3 lysates; Human stomach and Human adenocarcinoma of colon tissues; HepG2 and HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14873
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/500.
Flow Cyt 1/260.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization
    Cell membrane. Golgi apparatus. Cytoplasm > perinuclear region. Cell projection. Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP-bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA409338 antibody
    • MEL antibody
    • Mel transforming oncogene (derived from cell line NK14) RAB8 homolog antibody
    • Mel transforming oncogene (derived from cell line NK14) antibody
    • Mel transforming oncogene (RAB8 homolog) antibody
    • Mel transforming oncogene antibody
    • MGC124948 antibody
    • Oncogene c mel antibody
    • Oncogene c-mel antibody
    • RAB8 antibody
    • RAB8A antibody
    • RAB8A member RAS oncogene family antibody
    • RAB8A_HUMAN antibody
    • Ras associated protein RAB8 antibody
    • Ras related protein Rab 8A antibody
    • Ras-related protein Rab-8A antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: RAB8A knockout HAP1 cell lysate (20 µg)
    Lane 3: HCT116 cell lysate (20 µg)
    Lane 4: NIH/3T3 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab188574 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab188574 was shown to specifically react with RAB8A when RAB8A knockout samples were used. Wild-type and RAB8A knockout samples were subjected to SDS-PAGE. ab188574 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of colon tissue labelling RAB8A with ab188574 at 1/500. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Counter stained with Hematoxylin.

  • All lanes : Anti-RAB8A antibody [EPR14873] (ab188574) at 1/1000 dilution

    Lane 1 : C6 lysate
    Lane 2 : RAW264.7 lysate
    Lane 3 : PC-12 lysate
    Lane 4 : NIH3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa

  • Anti-RAB8A antibody [EPR14873] (ab188574) at 1/1000 dilution + HCT-116 lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 24 kDa
    Observed band size: 24 kDa

  • All lanes : Anti-RAB8A antibody [EPR14873] (ab188574) at 1/10000 dilution

    Lane 1 : HepG2 lysate
    Lane 2 : HeLa lysate
    Lane 3 : 293T lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa

  • Flow Cytometrical analysis of HeLa cells labeling RAB8A with ab188574 at 1/260 (pink) compared to a Isotype control (green). FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 were used for the analysis.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labelling RAB8A with ab188574 at 1/500 followed by Goat anti rabbit IgG (Alexa Fluor®488) and DAPI staining.

  • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling RAB8A with ab188574 at 1/500. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Counter stained with Hematoxylin.

References

This product has been referenced in:
  • Madero-Pérez J  et al. Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation. Mol Neurodegener 13:3 (2018). Read more (PubMed: 29357897) »
  • Burikhanov R  et al. Chloroquine-Inducible Par-4 Secretion Is Essential for Tumor Cell Apoptosis and Inhibition of Metastasis. Cell Rep 18:508-519 (2017). Read more (PubMed: 28076793) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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