Overview

  • Product nameAnti-Rab9 antibody
    See all Rab9 primary antibodies
  • Description
    Mouse monoclonal to Rab9
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 17-116 of Human Rab9, with GST tag.

  • Positive control
    • HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab68340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 23 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionInvolved in the transport of proteins between the endosomes and the trans Golgi network.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
  • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410064E05Rik antibody
    • AI195561 antibody
    • DmRab9 antibody
    • Rab 9 antibody
    • RAB 9A antibody
    • RAB9 member RAS oncogene family antibody
    • RAB9A antibody
    • RAB9A member RAS oncogene family antibody
    • RAB9A_HUMAN antibody
    • RAS ASSOCIATED PROTEIN RAB9 antibody
    • Ras related protein Rab 9A antibody
    • Ras-related protein Rab-9A antibody
    • Sid6061p antibody
    • Sid99 antibody
    see all

Anti-Rab9 antibody images

  • Anti-Rab9 antibody (ab68340) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 23 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Anti-Rab9 antibody (ab68340) at 5 µg/ml + HeLa cell lysate at 25 µg/ml

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
    Additional bands at : 33 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence of monoclonal antibody to Rab9 ab68340 on HeLa cells. Antibody concentration 10 ug/ml.
  • Detection limit for recombinant GST tagged Rab9 is approximately 0.1ng/ml as a capture antibody.

References for Anti-Rab9 antibody (ab68340)

ab68340 has not yet been referenced specifically in any publications.

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