Recombinant
RabMAb

Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)

Overview

  • Product name
    Anti-Rab9 antibody [EPR13272] - Late Endosome Marker
    See all Rab9 primary antibodies
  • Description
    Rabbit monoclonal [EPR13272] to Rab9 - Late Endosome Marker
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Rab9 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P51151

  • Positive control
    • K562, HeLa, 293T and HepG2 cell lysates; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
ICC/IF 1/250.

For unpurified, use 1/50 - 1/100.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    Images

    • Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815) at 1/2000 dilution (purified) + rat kidney at 10 µg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size: 23 kDa
      Observed band size: 23 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815) at 1/2000 dilution (purified) + mouse spleen at 20 µg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 23 kDa
      Observed band size: 23 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815) at 1/2000 dilution (purified)

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 23 kDa
      Observed band size: 23 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunofluorescence staining of HepG2 cells with purified ab179815 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab179815 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Immunofluorescent staining of HepG2 cells labeling Rab9 with unpurified ab179815 at 1/50 dilution (red). DAPI nuclear staining (blue).

    • All lanes : Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815) at 1/1000 dilution (unpurified)

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 23 kDa

    References

    This product has been referenced in:
    • Uchiyama K  et al. Prions amplify through degradation of the VPS10P sorting receptor sortilin. PLoS Pathog 13:e1006470 (2017). Read more (PubMed: 28665987) »
    • Kim SW  et al. Covalent, Non-Covalent, Encapsulated Nanodrug Regulate the Fate of Intra- and Extracellular Trafficking: Impact on Cancer and Normal Cells. Sci Rep 7:6454 (2017). Read more (PubMed: 28743942) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    HEK293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 24 2015

    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (Brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 24 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (bEnd.3)
    Permeabilization
    Yes - 0.1% Triton in PBS
    Specification
    bEnd.3
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 03 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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