Rabbit Anti-Chicken IgY H&L (Alkaline Phosphatase) (ab97137)
Key features and details
- Rabbit Anti-Chicken IgY H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Rabbit Anti-Chicken IgY H&L (Alkaline Phosphatase)
See all IgY secondary antibodies -
Host species
Rabbit -
Target species
Chicken -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP). -
Conjugation notes
Molar enzyme: antibody protein ratio is 1:1 -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Part of the AbExcel range. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/100 - 1/1000.
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ELISA |
1/5000 - 1/50000. (as Primary).
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|
WB |
1/5000 - 1/50000. (Chemiluminescent).
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Notes |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/100 - 1/1000. |
ELISA
1/5000 - 1/50000. (as Primary). |
WB
1/5000 - 1/50000. (Chemiluminescent). |
Images
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All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Rabbit Anti-Chicken IgY H&L (Alkaline Phosphatase) (ab97137) at 1/5000 dilution
Lanes 3-4 : Rabbit Anti-Chicken IgY H&L (Alkaline Phosphatase) (ab97137) at 1/20000 dilution
Lanes 5-6 : Rabbit Anti-Chicken IgY H&L (Alkaline Phosphatase) (ab97137) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 12 minutes
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab97137 has not yet been referenced specifically in any publications.