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Our Abpromise guarantee covers the use of ab97137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|IHC-P||1/100 - 1/1000.|
|ELISA||1/5000 - 1/50000. (as Primary).|
|WB||1/5000 - 1/50000. (Chemiluminescent).|
All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AP) (ab97137) at 1/5000 dilution
Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AP) (ab97137) at 1/20000 dilution
Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AP) (ab97137) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time : 12 minutes
ab97137 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"