AbExcel

Rabbit Anti-Chicken IgY H&L (Alkaline Phosphatase) (ab97137)

Overview

  • Product nameRabbit Anti-Chicken IgY H&L (Alkaline Phosphatase)
    See all IgY secondary antibodies
  • Target speciesChicken
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applicationsICC, IHC-P, ELISA, WBmore details
  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • Conjugation notesMolar enzyme: antibody protein ratio is 1:1
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesPart of the AbExcel range.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/100 - 1/1000.
ELISA 1/5000 - 1/50000. (as Primary).
WB 1/5000 - 1/50000. (Chemiluminescent).

Rabbit Anti-Chicken IgY H&L (Alkaline Phosphatase) images

  • All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AP) (ab97137) at 1/5000 dilution
    Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AP) (ab97137) at 1/20000 dilution
    Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AP) (ab97137) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time : 12 minutes

References for Rabbit Anti-Chicken IgY H&L (Alkaline Phosphatase) (ab97137)

ab97137 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab97137.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"