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Shipped at 4°C. Store at +4°C.
Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
Concentration information loading...
Immunogen affinity purified
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
Molar enzyme: antibody protein ratio is 1:1
Part of the
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
1/100 - 1/1000.
1/5000 - 1/50000. (as Primary).
1/5000 - 1/50000. (Chemiluminescent).
Rabbit Anti-Chicken IgY H&L (Alkaline Phosphatase) images
Western blot - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AP) (ab97137)
All lanes :
Anti-beta Actin antibody - Loading Control (
) at 1 µg/ml
Lanes 1-6 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AP) (ab97137) at 1/5000 dilution Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AP) (ab97137) at 1/20000 dilution Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AP) (ab97137) at 1/50000 dilution Developed using the ECL technique. Performed under reducing conditions. Exposure time : 12 minutes
References for Rabbit Anti-Chicken IgY H&L (Alkaline Phosphatase) (ab97137)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"