Rabbit Anti-Chicken IgY H&L (DyLight® 488) preadsorbed (ab96955)

Overview

  • Product nameRabbit Anti-Chicken IgY H&L (DyLight® 488) preadsorbed
    See all IgY secondary antibodies
  • Target speciesChicken
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat was detected.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity

    Cow, Goat, Horse, Human, Mouse, Pig, Rabbit, Rat more details
  • ConjugationDyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using bovine, goat, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • ClonalityPolyclonal
  • IsotypeIgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Rabbit Anti-Chicken IgY H&L (DyLight® 488) preadsorbed images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Rabbit Anti-Chicken IgY H&L (DyLight® 488) preadsorbed (ab96955)

ab96955 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply. A549 cells were recommended because they showed weak to no staining using a similar antibody.  Plus these are the results found in the Human Protein Atlas: http://www.proteinatlas.org/ENSG00000169252/cell/A-549 I'm not sure if...

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Thank you for your reply and for providing the requested information. After seeing the data and your protocol, I would like to make some suggestions that may improve your results. One of my colleagues who has worked with chicken antibodies in the p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"