Overview

  • Product name
    Rabbit Anti-Chicken IgY H&L (FITC)
    See all IgY secondary antibodies
  • Target species
    Chicken
  • Tested applications
    Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details
  • Immunogen

    chicken IgY whole molecule

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000. PubMed: 18983843
ELISA 1/10000 - 1/50000.

References for Rabbit Anti-Chicken IgY H&L (FITC) (ab6749)

This product has been referenced in:
  • Buijs PR  et al. Different responses of human pancreatic adenocarcinoma cell lines to oncolytic Newcastle disease virus infection. Cancer Gene Ther 21:24-30 (2014). Read more (PubMed: 24384773) »
  • Na GH  et al. Effects of glucose concentration in the medium on rat hepatocyte culture. Ann Surg Treat Res 87:53-60 (2014). ICC/IF . Read more (PubMed: 25114883) »

See all 7 Publications for this product

Product Wall

Thank you for your enquiry. ab6749, ab7114 and ab6873 are all suitable for flow cytometry analysis, have all been raised against chicken IgY and are all FITC conjugated. There is very little difference between the three aside from the mass of antib...

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Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet. We do not have a specific protocol for this antibody in immunofluorescence, or a specific reference. If you have any more questions...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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