Overview

  • Product name
    Rabbit Anti-Chicken IgY H&L (HRP)
    See all IgY secondary antibodies
  • Target species
    Chicken
  • Tested applications
    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    chicken IgY whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Gentamicin Sulfate (Do not add Sodium Azide!)
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Horseradish Peroxidase (HRP).
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • General notes

    May cross-react with chicken IgM

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent concentration.
ELISA 1/145000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/2500 - 1/10000.

Rabbit Anti-Chicken IgY H&L (HRP) images

  • Ab6753 was used at dilution 1/2000 with the primary antibody ab16293 in IHC-Fr. See the review on ab16293.
  • Ab6753 was used at dilution 1/10000 with the primary antibody ab15879 in ELISA. See the review on ab15879.
  • Ab6753 was used at dilution 1/5000 with the primary antibody ab19130 in WB. See the review on ab19130.

References for Rabbit Anti-Chicken IgY H&L (HRP) (ab6753)

This product has been referenced in:
  • Mihic-Probst D  et al. Tumor cell plasticity and angiogenesis in human melanomas. PLoS One 7:e33571 (2012). IHC-P . Read more (PubMed: 22442699) »
  • Vergnes L  et al. Heart-type Fatty Acid-binding Protein Is Essential for Efficient Brown Adipose Tissue Fatty Acid Oxidation and Cold Tolerance. J Biol Chem 286:380-90 (2011). IHC-Fr . Read more (PubMed: 21044951) »

See all 12 Publications for this product

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I have had the opportunity to speak with my collegues regarding this issue.We have seen similar patterns when the gel is over-heating during transfer or contaminants are present in the transfer buffer or equipm...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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