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Product name Rabbit Anti-Chicken IgY H&L (HRP) See all IgY secondary antibodies
Target species Chicken
Specificity By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Tested applications Suitable for:
ICC, IHC-P, ELISA, WB more details
Conjugation HRP Properties
Storage instructions Shipped at 4°C. Store at +4°C.
Storage buffer Preservative: 0.1% Proclin
Constituents: 0.2% BSA, PBS
Concentration information loading...
Purity Immunogen affinity purified
Purification notes This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
Conjugation notes Molar enzyme/ antibody protein ratio is 4:1
General notes Part of the AbExcel range.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
1/200 - 1/5000.
1/10000 - 1/100000. (Primary).
1/2000 - 1/20000. Colorimetric: Use 1/5,000-1/30,000, Chemiluminescent: Use 1/10,000-1/50,000
Rabbit Anti-Chicken IgY H&L (HRP) images
Western blot - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140)
All lanes :
Anti-beta Actin antibody - Loading Control (
) at 1 µg/ml
Lanes 1-6 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/2000 dilution Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/10000 dilution Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/20000 dilution Developed using the ECL technique Performed under reducing conditions. Exposure time : 1 minute
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"