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Our Abpromise guarantee covers the use of ab97140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|IHC-P||1/200 - 1/5000.|
|ELISA||1/10000 - 1/100000. (Primary).|
|WB||1/2000 - 1/20000. Colorimetric: Use 1/5,000-1/30,000, Chemiluminescent: Use 1/10,000-1/50,000|
All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/2000 dilution
Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/10000 dilution
Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/20000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"