Rabbit Anti-Dog IgG H&L (Alkaline Phosphatase) (ab49697)

Overview

  • Product name
    Rabbit Anti-Dog IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Description
    Rabbit polyclonal Secondary Antibody to Dog IgG - H&L (AP)
  • Target species
    Dog
  • Specificity
    This antibody reacts with canine IgG. (Specificity is determined by immunoelectrophoresis and double diffusion assays prior to conjugation).
  • Tested applications
    Suitable for: ELISA, Dot blot, WBmore details
  • Immunogen

    Normal dog serum.

  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 8.00
    Preservative: 0.097% Sodium azide
    Constituents: 0.0095% Magnesium chloride, 0.605% Tris, 1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    ab49697 is isolated from pooled normal dog serum, the antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction.
  • Conjugation notes
    Conjugated to Alkaline Phosphatase using 0.2% glutaraldehyde.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Dot blot
WB
  • Application notes
    Dot: 1/4000.
    ELISA: 1/4000.
    WB (chemiluminescent): 1/4000. Predicted molecular weight: 36 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    ab49697 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Gracias por tu respuesta.

    En el siguiente link encontrarás todos los secundarios disponibles anti-Dog.

    http://www.abcam.com/index.html?c=4849

    Me temo que no tenemos ninguno monoclonal, pero mi compañera respo...

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    Gracias por tu respuesta.

    El anticuerpo al que te refieres, el ab49697 no está pre absorbido. Durante su purificación el antisuero se ha fraccionado y purificado por cromatografía de intercambio iónico para obtener...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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