Rabbit F(ab) Anti-Cow IgG H&L (FITC) (ab136728)

Overview

  • Product name
    Rabbit F(ab) Anti-Cow IgG H&L (FITC)
    See all IgG secondary antibodies
  • Description
    Rabbit F(ab) polyclonal Secondary Antibody to Cow IgG - H&L (FITC)
  • Host species
    Rabbit
  • Target species
    Cow
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, ICC, ICC/IF, Flow Cytmore details
  • Immunogen

    Highly purified whole bovine immunoglobulin

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Fab and F(ab')2 are respectively mono and bivalent fragments of immunoglobulins, i.e. including one or two sites for the fixation of the antigen. They are prepared by enzymic digestion (papain or pepsin). They are purified by affinity chromatography and are thus particularly specific.
    Fab and F(ab')2 fragments have certain advantages with respect to the entire antibodies in immunohistochemistry, immunocytology and flow cytometry tests.

    On the one hand their specificity is increased since they can no longer be bound in a non-specific way by their Fc part as entire Ig’s are.

    On the other hand their use also increase the sensitivity of the technique. In fact, their small size enhances their penetration into tissues and cells as well as their availability for the antigens (this is so particularly for Fab).

    Finally, conjugate Fab\'s have a label/antigenic ratio that is twice as much compared to antibodies (except fluorescent conjugates).
  • Conjugation notes
    The F/P (fluorochrome/protein) molar ratio is set between 3 and 7.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC 1/10 - 1/100.
ICC/IF 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration.

References

ab136728 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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