Rabbit F(ab')2 Anti-Goat IgG - H&L (AP), pre-adsorbed (ab5756)

Overview

  • Product name
    Rabbit F(ab')2 Anti-Goat IgG - H&L (AP), pre-adsorbed
    See all IgG secondary antibodies
  • Target species
    Goat
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
  • Tested applications
    Suitable for: ICC/IF, Dot blot, WB, ELISA, IHCmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Goat IgG whole molecule

  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 8.00
    Preservative: 0.1% Sodium azide
    Constituents: 0.0001% Zinc chloride, 0.01% Magnesium chloride, 0.788% Tris HCl, 0.876% Sodium chloride, 1% BSA
    Note: BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/2000.
Dot blot 1/5000 - 1/50000.
WB 1/500 - 1/5000.
ELISA 1/10000 - 1/40000.
IHC Use at an assay dependent dilution.

References

ab5756 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab5756.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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