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Product name Rabbit F(ab')2 Anti-Goat IgG - H&L (Biotin), pre-adsorbed See all IgG secondary antibodies
Target species Goat
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human Serum Proteins.
Tested applications Suitable for:
WB, ICC/IF, Dot blot, ELISA, IHC, Immunomicroscopy, Flow Cyt more details
Minimal cross-reactivity Human
To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
Goat IgG whole molecule
Conjugation Biotin Properties
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.05% Sodium Azide
Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration information loading...
Purification notes This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notes Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000.
1/1000 - 1/5000.
1/20000 - 1/100000.
1/500 - 1/5000.
Use at an assay dependent dilution.
1/200 - 1/1000.
References for Rabbit F(ab')2 Anti-Goat IgG - H&L (Biotin), pre-adsorbed (ab5753)
This product has been referenced in:
et al. Murine mesenchymal stem cells exhibit a restricted repertoire of functional chemokine receptors: comparison with human. PLoS ONE 3:e2934 (2008).
Read more (PubMed: 18698345) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"