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Product name Rabbit F(ab')2 Anti-Goat IgG H&L (FITC) See all IgG secondary antibodies
Target species Goat
Specificity This antibody reacts with Goat heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-pepsin.
Tested applications Suitable for:
Flow Cyt, ICC/IF more details
Full length (Goat) IgG whole molecules.
Conjugation FITC. Ex: 493nm, Em: 528nm Properties
Storage instructions Shipped at 4°C. Store at +4°C.
Storage buffer Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Concentration information loading...
Purity Immunogen affinity purified
Purification notes Prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notes 4.1 moles FITC per mole of Rabbit IgG F(ab') 2
Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons)
Absorption Wavelength: 495 nm; Emission Wavelength: 528 nm.
This antibody is suitable for antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000.
1/500 - 1/2500.
References for Rabbit F(ab')2 Anti-Goat IgG H&L (FITC) (ab47846)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"