Rabbit F(ab')2 Anti-Goat IgG H&L (FITC) (ab47846)

Overview

  • Product name
    Rabbit F(ab')2 Anti-Goat IgG H&L (FITC)
    See all IgG secondary antibodies
  • Target species
    Goat
  • Specificity
    This antibody reacts with Goat heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-pepsin.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IFmore details
  • Immunogen

    Full length (Goat) IgG whole molecules.

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes
    4.1 moles FITC per mole of Rabbit IgG F(ab')2 Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm; Emission Wavelength: 528 nm.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes


    This antibody is suitable for antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2000 - 1/10000.
ICC/IF 1/500 - 1/2500.

References

ab47846 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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