Rabbit F(ab')2 Anti-Goat IgG H&L (FITC) (ab47846)

Overview

  • Product nameRabbit F(ab')2 Anti-Goat IgG H&L (FITC)
    See all IgG secondary antibodies
  • Target speciesGoat
  • SpecificityThis antibody reacts with Goat heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-pepsin.
  • Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
  • Immunogen

    Full length (Goat) IgG whole molecules.

  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPrepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes4.1 moles FITC per mole of Rabbit IgG F(ab')2 Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm; Emission Wavelength: 528 nm.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notes


    This antibody is suitable for antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2000 - 1/10000.
ICC/IF 1/500 - 1/2500.

References for Rabbit F(ab')2 Anti-Goat IgG H&L (FITC) (ab47846)

ab47846 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"