Rabbit F(ab')2 Anti-Goat IgG - H&L (HRP), pre-adsorbed (ab5755)
Key features and details
- Rabbit F(ab')2 Anti-Goat IgG - H&L (HRP), pre-adsorbed
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC
Overview
-
Product name
Rabbit F(ab')2 Anti-Goat IgG - H&L (HRP), pre-adsorbed
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Goat -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins. -
Tested applications
Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details -
Minimal
cross-reactivity
Human more details -
Immunogen
Goat IgG whole molecule.
-
Conjugation
HRP
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading...
-
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/500 - 1/2500.
|
|
Dot blot |
Use at an assay dependent dilution.
|
|
WB |
1/1000 - 1/10000.
|
|
ELISA |
1/10000 - 1/50000. This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
|
|
Electron Microscopy |
Use at an assay dependent dilution.
|
|
IHC |
Use at an assay dependent dilution.
|
Notes |
---|
ICC/IF
1/500 - 1/2500. |
Dot blot
Use at an assay dependent dilution. |
WB
1/1000 - 1/10000. |
ELISA
1/10000 - 1/50000. This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. |
Electron Microscopy
Use at an assay dependent dilution. |
IHC
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (3)
ab5755 has been referenced in 3 publications.
- Li Q et al. Poricoic acid A suppresses TGF-ß1-induced renal fibrosis and proliferation via the PDGF-C, Smad3 and MAPK pathways. Exp Ther Med 21:289 (2021). PubMed: 33717232
- Díaz-Jiménez D et al. A functional IL1RL1 variant regulates corticosteroid-induced sST2 expression in ulcerative colitis. Sci Rep 7:10180 (2017). PubMed: 28860510
- Belian E et al. Forward Programming of Cardiac Stem Cells by Homogeneous Transduction with MYOCD plus TBX5. PLoS One 10:e0125384 (2015). WB . PubMed: 26047103