Prepared from monospecific antiserum by immunoaffinity chromatography using goat IgG coupled to agarose beads followed by pepsin digesion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody.
Conjugated to R-Phycoerythrin (R-PE) (MW 240kD) from seaweed.
Absorption Wavelength: 490 nm, 545 nm and 565 nm
Emission Wavelength: 580 nm