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Product name Rabbit F(ab')2 Anti-Goat IgG H&L (TRITC) preadsorbed See all IgG secondary antibodies
Target species Goat
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
Tested applications Suitable for:
ICC/IF, Immunomicroscopy, Flow Cyt, ELISA more details
Minimal cross-reactivity Human
To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
Goat IgG whole molecule.
Conjugation TRITC. Ex: 547nm, Em: 572nm Properties
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.164% Sodium phosphate, 1.45% Sodium chloride, 1% BSA BSA is IgG and protease free
Concentration information loading...
Purification notes This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notes Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons)
Absorption Wavelength: 550 nm
Emission Wavelength: 570 nm
Fluorochrome/Protein Ratio: 1.6 moles TRITC per mole of Rabbit IgG F(ab’)2
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000.
Use at an assay dependent dilution.
1/500 - 1/2500.
1/10000 - 1/50000.
References for Rabbit F(ab')2 Anti-Goat IgG H&L (TRITC) preadsorbed (ab5752)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"