Rabbit F(ab')2 Anti-Mouse IgG - H&L (AP), pre-adsorbed (ab98774)

Overview

  • Product nameRabbit F(ab')2 Anti-Mouse IgG - H&L (AP), pre-adsorbedSee all IgG secondary antibodies ...
  • Target speciesMouse
  • Tested applicationsICC, IHC-P, ELISA, WB more details
  • Minimal
    cross-reactivity

    Human, Rat more details
  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Alkaline Phosphatase.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab98774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC Use at an assay dependent dilution.
IHC-P 1/100 - 1/1000.
ELISA 1/1000 - 1/10000. (as Primary).
WB 1/1000 - 1/10000. (Chemiluminescent).

References for Rabbit F(ab')2 Anti-Mouse IgG - H&L (AP), pre-adsorbed (ab98774)

ab98774 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"