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Product name Rabbit F(ab')2 Anti-Mouse IgG - H&L (FITC), pre-adsorbed See all IgG secondary antibodies
Target species Mouse
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
Tested applications Suitable for:
ICC/IF, Immunomicroscopy, Flow Cyt, ELISA more details
Minimal cross-reactivity Human
To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
Mouse IgG whole molecule
Conjugation FITC. Ex: 493nm, Em: 528nm Properties
Storage instructions Shipped at 4°C. Store at +4°C.
Storage buffer Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration information loading...
Purification notes This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notes Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons)
Absorption Wavelength: 495 nm Emission Wavelength: 528 nm
Fluorochrome/Protein Ratio: 2.6 moles FITC per mole of Rabbit IgG F(ab')2
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000.
Use at an assay dependent dilution.
1/500 - 1/2500.
1/10000 - 1/50000.
References for Rabbit F(ab')2 Anti-Mouse IgG - H&L (FITC), pre-adsorbed (ab5758)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"