Rabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed (ab7000)

Overview

  • Product nameRabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesMouse
  • Tested applicationsSuitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Frmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Full length native Mouse IgG (purified).

  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPrepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • Conjugation notesR-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • ClonalityPolyclonal
  • General notes


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Immunomicroscopy 1/50 - 1/200.
Flow Cyt 1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 mg of antibody conjugate.
IHC-P 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.

References for Rabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed (ab7000)

ab7000 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"