Overview

  • Product nameRabbit F(ab')2 Anti-Rat IgG H&L
    See all IgG secondary antibodies
  • Target speciesRat
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
  • Tested applicationsIHC-P, ELISA, WB, ICCmore details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab102200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/2000.
ELISA 1/100 - 1/30000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000).
WB 1/1000 - 1/30000. (Colorimetric: 1/1000 - 1/10000; Chemiluminescent 1/1000 - 1/30000.
ICC 1/200 - 1/2000.

References for Rabbit F(ab')2 Anti-Rat IgG H&L (ab102200)

ab102200 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"