Rabbit F(ab')2 Anti-Rat IgG - H&L (AP), pre-adsorbed (ab6251)

Overview

  • Product nameRabbit F(ab')2 Anti-Rat IgG - H&L (AP), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesRat
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c), or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
  • Tested applicationsSuitable for: ICC/IF, ELISA, IHC, Dot blot, WBmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Mouse, Rabbit, Sheep more details
  • Immunogen

    Rat IgG whole molecule

  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 8.00
    Preservative: 0.05% Sodium azide
    Constituents: 0.788% Tris HCl, 0.878% Sodium chloride, 1% BSA, 0.0095% Magnesium chloride, 0.014% Zinc chloride, 50% Glycerol

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
ELISA
IHC
Dot blot
WB
  • Application notesDot: Use at an assay dependent dilution.
    ELISA: 1/1000 - 1/4000.
    IHC: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/1000 to 1/4000 of the reconstitution concentration is suggested for this product.

    Suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Rabbit F(ab')2 Anti-Rat IgG - H&L (AP), pre-adsorbed (ab6251)

    ab6251 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab6251.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"