For the best experience on the Abcam website please upgrade to a modern browser such as
Product name Rabbit F(ab')2 Anti-Rat IgG - H&L (TRITC ), pre-adsorbed See all IgG secondary antibodies
Target species Rat
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
Tested applications Suitable for:
ICC/IF, Flow Cyt, Immunomicroscopy, ELISA more details
Minimal cross-reactivity Cow, Horse, Human, Mouse, Rabbit, Sheep
To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
Rat IgG whole molecule
Conjugation TRITC. Ex: 547nm, Em: 572nm Properties
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration information loading...
Purification notes This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notes Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons)
Absorption Wavelength: 550 nm
Emission Wavelength: 570 nm
Fluorochrome/Protein Ratio: 2.1 moles TRITC per mole of Rabbit IgG F(ab’)2
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes Flow Cyt: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Rabbit F(ab')2 Anti-Rat IgG - H&L (TRITC ), pre-adsorbed (ab6248)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"