Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (ab6742)


  • Product nameRabbit Anti-Goat IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Target speciesGoat
  • Tested applicationsSuitable for: Dot Blot, ELISA, ICC/IF, WB, IHC-P, IHC-Frmore details
  • Immunogen

    Goat IgG whole molecule

  • ConjugationAlkaline Phosphatase


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 8.00
    Preservative: 0.1% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads.
  • Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab6742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot Blot Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.
ICC/IF 1/200 - 1/1000.
WB Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) images

  • Ab6742 was used at dilution 1/4000 with the primary antibody ab20773 in ELISA. See the review on ab20773.

References for Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (ab6742)

This product has been referenced in:
  • Hughes AJ & Herr AE Microfluidic Western blotting. Proc Natl Acad Sci U S A : (2012). WB . Read more (PubMed: 23223527) »
  • Takayama H  et al. High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS ONE 3:e2314 (2008). ELISA . Read more (PubMed: 18523555) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

As discussed we have many anti goat antibodies that can be used in ELISA and ICC/IF.

Idealy enzyme conjugated antibodies e.g. HRP, Alkaline phosphatase are the best suitable antibodies in ELISA. However...

Read More

Thank you for your enquiry. I see no reason why you can't substitute in a different secondary antibody, but you want to consult the instructions of the kit that you have, or are interested in purchasing. If you have any more questions, please contact u...

Read More

Thank you for sending the details of your protocol and the picture of your blot. I really think that the background bands are due to the secondary. What appears to be happening is that the secondary is binding additional proteins and reacting with the ...

Read More