Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbed (ab7133)

Overview

  • Product name
    Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbed
    See all IgG secondary antibodies
  • Target species
    Goat
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Goat IgG whole molecule

  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.79% Tris HCl, 0.0095% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads.
  • Conjugation notes
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB Use at an assay dependent dilution.
ICC/IF 1/200 - 1/1000.

References for Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbed (ab7133)

ab7133 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. 1. How much lysate was loaded? We would suggest 5uL for 10x8cm gel. 2. Try to titer up the secondary antibody. The problem seems like a non-specific binding caused by too much antigen and/or antibody.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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