Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbed (ab7133)

Overview

  • Product nameRabbit Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbedSee all IgG secondary antibodies ...
  • Description
    Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP), pre-adsorbed
  • Target speciesGoat
  • Tested applicationsDot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Goat IgG whole molecule

  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.79% Tris HCl, 0.0095% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads.
  • Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab7133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot Blot Use at an assay dependent concentration.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB Use at an assay dependent dilution.
ICC/IF 1/200 - 1/1000.

References for Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbed (ab7133)

ab7133 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. 1. How much lysate was loaded? We would suggest 5uL for 10x8cm gel. 2. Try to titer up the secondary antibody. The problem seems like a non-specific binding caused by too much antigen and/or antibody.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"