Rabbit Anti-Goat IgG H&L (Biotin) (ab6740)
Key features and details
- Rabbit Anti-Goat IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WB
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Goat IgG H&L (Biotin)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Goat -
Tested applications
Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details -
Immunogen
Goat IgG whole molecule
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.88% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Dot blot | ||
ELISA | ||
IHC-P | ||
IHC-Fr | ||
Immunomicroscopy | ||
ICC/IF | ||
WB |
Notes |
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ELISA: Use at an assay dependent dilution.
IHC-P: 1/100 (PMID 18930986).
IHC-Fr: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab6740 has been referenced in 17 publications.
- Costa GMJ et al. High SARS-CoV-2 tropism and activation of immune cells in the testes of non-vaccinated deceased COVID-19 patients. BMC Biol 21:36 (2023). PubMed: 36797789
- Farias TO et al. Testis and brown adipose tissue xenografts from yellowish myotis (Myotis levis). Reprod Fertil 3:287-300 (2022). PubMed: 36331914
- Scheld M et al. Modulatory effect of 17ß-estradiol on myeloid cell infiltration into the male rat brain after ischemic stroke. J Steroid Biochem Mol Biol 202:105667 (2020). PubMed: 32407868
- de Oliveira CFA et al. Comparative testis structure and function in three representative mice strains. Cell Tissue Res 382:391-404 (2020). PubMed: 32666138
- Wang Z et al. TRPV4-induced inflammatory response is involved in neuronal death in pilocarpine model of temporal lobe epilepsy in mice. Cell Death Dis 10:386 (2019). PubMed: 31097691