Rabbit Anti-Goat IgG H&L (Biotin) preadsorbed (ab7131)

Overview

  • Product nameRabbit Anti-Goat IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies
  • Target speciesGoat
  • Tested applicationsSuitable for: ICC/IF, Dot blot, ELISA, IHC-P, Immunomicroscopy, WB, Flow Cyt, IHC-Frmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Goat IgG whole molecule

  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • ClonalityPolyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution. PubMed: 20838433
IHC-Fr Use at an assay dependent concentration.

References for Rabbit Anti-Goat IgG H&L (Biotin) preadsorbed (ab7131)

This product has been referenced in:
  • Fernandez P  et al. Strain-transcendent immune response to recombinant Var2CSA DBL5-e domain block P. falciparum adhesion to placenta-derived BeWo cells under flow conditions. PLoS One 5:e12558 (2010). Flow Cyt . Read more (PubMed: 20838433) »

See 1 Publication for this product

Product Wall

Thank you for your message and for providing this further information.

Reviewing the details, I agree there seems to be some crossreactivity to mouse IgG. I can confirm that this antibody has not specifically been pre absorbedwith mouse, so ...

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Thank you for your telephone call yesterday afternoon. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody and have some concerns about crossreactivity to mouse..

I would like to reassure you that thi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"