Rabbit Anti-Goat IgG H&L (DyLight® 550) preadsorbed (ab96916)

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Overview

  • Product nameRabbit Anti-Goat IgG H&L (DyLight® 550) preadsorbed
    See all IgG secondary antibodies
  • Description
    Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 550), pre-adsorbed
  • Target speciesGoat
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity
    Chicken, Horse, Human, Mouse, Pig, Rat more details
  • ConjugationDyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notesDyLight® 550 replaces DyLight® 549.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500. DyLight®550 replaces DyLight®549. The DyLight®550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Rabbit Anti-Goat IgG H&L (DyLight® 550) preadsorbed images

  • DyLight®-Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 550), pre-adsorbed(ab96916)
    DyLight®-Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 550), pre-adsorbed(ab96916)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Rabbit Anti-Goat IgG H&L (DyLight® 550) preadsorbed (ab96916)

ab96916 has not yet been referenced specifically in any publications.

Publishing research using ab96916? Please let us know so that we can cite the reference in this datasheet.

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Inquiry: Product purchased at Dec20, order number: 1227684 Cat: Ab27985 Cat: ab96916 We purchased the two anbodies at the same order, our result shows high background and low signal. We are not sure which one gave us problem, or which step we...

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Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab27985 and ab96916.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"