Rabbit Anti-Goat IgG H&L (FITC) preadsorbed (ab7128)

Overview

  • Product nameRabbit Anti-Goat IgG H&L (FITC) preadsorbed
    See all IgG secondary antibodies
  • Target speciesGoat
  • Tested applicationsSuitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISA, IHC-P, IHC-Frmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Goat IgG whole molecule

  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesDiaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles DTAF per mole of Rabbit IgG
  • ClonalityPolyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Immunomicroscopy
Flow Cyt
ELISA
IHC-P
IHC-Fr
  • Application notesSuitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
    For FACS analysis, it is useful to do titration for this secondary antibody: 1:1000, 1:2500 and 1:5000 and the blocking buffer should be 3% BSA.
  • References for Rabbit Anti-Goat IgG H&L (FITC) preadsorbed (ab7128)

    ab7128 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab7128.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"