Overview

  • Product nameRabbit Anti-Goat IgG H&L (HRP)
    See all IgG secondary antibodies
  • Target speciesGoat
  • Tested applicationsSuitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
  • Immunogen

    Goat IgG whole molecule

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesHorseradish Peroxidase (HRP)
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
ELISA 1/175000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
WB 1/5000 - 1/20000. PubMed: 17369188

Rabbit Anti-Goat IgG H&L (HRP) images

  • Ab6741 was used at dilution 1/5000 with the primary antibody ab23938 in WB. See the review on ab23938.
  • Ab6741 was used at dilution 1/1000 with the primary antibody ab2247 in ELISA. See the review on ab2247.

References for Rabbit Anti-Goat IgG H&L (HRP) (ab6741)

This product has been referenced in:
  • Munday DC  et al. Interactome analysis of the human respiratory syncytial virus RNA polymerase complex identifies protein chaperones as important cofactors that promote L-protein stability and RNA synthesis. J Virol 89:917-30 (2015). WB ; Human . Read more (PubMed: 25355874) »
  • Hansen HG  et al. Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells. Sci Rep 5:18016 (2015). WB . Read more (PubMed: 26657798) »

See all 19 Publications for this product

Product Wall

We have recently concluded a stability study for conjugated antibodies. Based on the results of this study we have decided to issue the following guidelines: Biotin conjugates +4°C short term. Aliquot and store at -20°C. Avoid freeze/thaw...

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Thank you for contacting us.

Here are the links for the MSDS for the 2 products:

For ab6741: http://www.abcam.com/ps/Products/6/ab6741/Msds/ab6741_WPNA_EN.pdf
For ab8229: http://www.abcam.com/ps/Products/8/ab8229/Msds/ab8229_WPNA_...

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Thank you for contacting us.

Please check the datasheet and selct on basis of conc, price and volume. The compatible products are ab102345, ab102354, ab5755, ab97100, ab97105 and ab6741.

I hope this information is helpful to you. ...

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Thank you for your email. I am sorry to hear that you are experiencing problems with these antibodies.

The brown precipitates with HRP conjugated antibodies are very common. We recommend customers to pipette up and down and vortexing the vial...

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Thank you for your reply.

I was wondering if ab8227 would be a good replacement for you:

http://www.abcam.com/beta-Actin-antibody-Loading-Control-ab8227.html



If it is, then please let me know and I will get it s...

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Thank you for your reply.

I am sorry the replacement secondary did not solve this problem. Do you get the same non-specific binding pattern when you do your secondary only control? I ask, because if you do, then that does indicate that the is...

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Thank you for your reply.

As ab6741 did not prove to be successful for you, I am sending you ab7125 as a free of charge replacement. Your ne order number is ******* and you should have the new product by 10.30am tomorrow morning.

If...

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Thank you for your reply.

I am sorry that you are continuing to have problems with ab6741 in western blot. Therefore I would like to send you ab7125 as a free of charge replacement:

http://www.abcam.com/Donkey-polyclonal-Secondary-A...

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Thank you for contacting Abcam earlier today.

If you are still experiencing any issues with ab6741 in western blot after trying the protocol tips that we discussed, please let me know and I will be happy to look into sending a new antibody to...

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Thank you for your reply.

I can confirm that for the HRP conjugated antibodies the approach that Irecommended is the correct one. Spining the vial down will prevent free HRP to bind to un-specifically to samples and therefore avoid increased ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"