Rabbit Anti-Goat IgG H&L preadsorbed (ab97101)
Key features and details
- Rabbit Anti-Goat IgG H&L preadsorbed
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Rabbit Anti-Goat IgG H&L preadsorbed
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Goat -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.
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Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Minimal
cross-reactivity
Chicken, Horse, Human, Mouse, Pig, Rat more details
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/2000.
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ELISA |
1/100 - 1/10000. ELISA-Coating 1:100 - 1:500; ELISA-Primary 1:1000 - 1:10,000
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WB |
1/1000 - 1/30000. with chemiluminescent detection.
WB with Colorimetric detection: 1/1000 - 1/10000
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Notes |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/2000. |
ELISA
1/100 - 1/10000. ELISA-Coating 1:100 - 1:500; ELISA-Primary 1:1000 - 1:10,000 |
WB
1/1000 - 1/30000. with chemiluminescent detection. WB with Colorimetric detection: 1/1000 - 1/10000 |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab97101 has not yet been referenced specifically in any publications.