Rabbit Anti-Goat IgG+IgM+IgA H&L (FITC) (ab8514)

Overview

  • Product name
    Rabbit Anti-Goat IgG+IgM+IgA H&L (FITC)
    See all IgG + IgM + IgA secondary antibodies
  • Target species
    Goat
  • Specificity
    Antibodies were raised in rabbit to goat IgG, IgM and IgA
  • Tested applications
    Suitable for: ICC/IFmore details
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Specific antibodies were absorpted by incubation with Sepharose-bound goat immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized goat immunoglobulins a minimum of 65% protein bound.
  • Conjugation notes
    The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • Clonality
    Polyclonal
  • General notes
    F/P ratio is 3-5 mol Fluorescein per 1 mol of rabbit Ig. There is low background fluorescence of normal tissue in contrast to specific staining of sections treated with goat anti-human fibrogen antibodies
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notes
    For immunohistochemical staining of human tissue sections a 1:40 dilution is recommended.
  • References

    ab8514 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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