Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
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Immunogen affinity purified
Specific antibodies were absorpted by incubation with Sepharose-bound Guinea Pig immunoglobulins.
Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis.
After repeated binding with immobilized Guinea Pig immunoglobulins a minimum of 65% protein bound.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Recommended dilution as secondary antibody in ELISA is 1:1000.
Recommended dilution as secondary antibody in dot-blotting is 1:100.
References for Rabbit Anti-Guinea pig IgG+IgM+IgA H&L (ab8522)
This product has been referenced in:
Haase TN et al. Growth arrest specific protein (GAS) 6: a role in the regulation of proliferation and functional capacity of the perinatal rat beta cell. Diabetologia56:763-73 (2013).
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