Rabbit Anti-Horse IgG H&L (Alkaline Phosphatase) (ab49615)

Overview

  • Product nameRabbit Anti-Horse IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Target speciesHorse
  • Tested applicationsSuitable for: WB, ELISA, Dot blotmore details
  • Immunogen

    Purified horse IgG

  • Positive controlHorse IgG
  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 50% Glycerol, 1% BSA, 0.05M Tris buffered saline, 1mM Magnesium chloride, pH 8.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Conjugation notesRabbit anti-horse IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Dot blot
  • Application notesDot: 1/30000. Diluted conjugate detects up to 20 ng horse IgG
    bound to nitrocellulose.
    ELISA: 1/30000.
    WB: 1/30000. Predicted molecular weight: 36 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Rabbit Anti-Horse IgG H&L (Alkaline Phosphatase) (ab49615)

    ab49615 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"