Rabbit Anti-Human IgA alpha chain (Biotin) (ab97218)

Overview

  • Product nameRabbit Anti-Human IgA alpha chain (Biotin)
    See all IgA secondary antibodies
  • Target speciesHuman
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
  • Conjugation notesBiotin/ antibody protein ratio is 4:1
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/2500.
ICC/IF 1/100 - 1/500.
ELISA 1/10000 - 1/200000. (as Primary).
WB 1/10000 - 1/200000. (Chemiluminescent).

References for Rabbit Anti-Human IgA alpha chain (Biotin) (ab97218)

This product has been referenced in:
  • Hammar O  et al. Autoantibodies and gastrointestinal symptoms in infertile women in relation to in vitro fertilization. BMC Pregnancy Childbirth 13:201 (2013). ELISA . Read more (PubMed: 24191889) »

See 1 Publication for this product

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The laboratory has not tested these antibodies inparallel nor have they measured the affinity constants. WE are therefore not able to tell you which antibody would have higher affinity. I can howeverreassure you,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"