Rabbit Anti-Human IgA alpha chain (Biotin) (ab97218)

Overview

  • Product name
    Rabbit Anti-Human IgA alpha chain (Biotin)
    See all IgA secondary antibodies
  • Target species
    Human
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
  • Conjugation notes
    Biotin/ antibody protein ratio is 4:1
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/2500.
ICC/IF 1/100 - 1/500.
ELISA 1/10000 - 1/200000. (as Primary).
WB 1/10000 - 1/200000. (Chemiluminescent).

References

This product has been referenced in:
  • He X  et al. Upregulation of hyaluronan-mediated motility receptor in hepatocellular carcinoma predicts poor survival. Oncol Lett 10:3639-3646 (2015). Read more (PubMed: 26788183) »
  • Hammar O  et al. Autoantibodies and gastrointestinal symptoms in infertile women in relation to in vitro fertilization. BMC Pregnancy Childbirth 13:201 (2013). ELISA . Read more (PubMed: 24191889) »

See all 2 Publications for this product

Customer reviews and Q&As

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The laboratory has not tested these antibodies inparallel nor have they measured the affinity constants. WE are therefore not able to tell you which antibody would have higher affinity. I can howeverreassure you,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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