Rabbit Anti-Human IgA alpha chain (FITC) (ab97219)

Overview

  • Product name
    Rabbit Anti-Human IgA alpha chain (FITC)
    See all IgA secondary antibodies
  • Target species
    Human
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, Flow Cytmore details
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • Conjugation notes
    F/P ratio is 3-7
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

References

ab97219 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Flow Cytometry
Background: We wanted to distinguish human B cells into IgM+, IgG+ and IgA+ B cells by staining surface immunoglobulin. Since ab97219 is polyclonal, presence of an antibody targeting the extracellular part of the IgA heavy chain could be suspected but needed to be tested.
Staining: Human peripheral blood-derived PBMC stained with antibodies against CD3, CD19, surface IgG and surface IgA. In the image, lymphocytes are gated on CD3- CD19+
Results: There is a clear population of IgA+ B cells in the expected range. A few possibly IgA+/IgG+ B cells are present too.
Username

Mr. Marc Bigler

Verified customer

Submitted Aug 18 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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